TEST NAME & TAT |
CLINICAL CONDITION |
SPECIMEN TYPE |
COLLECTION |
PROCESSING |
SHIPPING |
Major t (9;22) quantitative by Real-Time PCR (14 days) |
Determination of t(9;22) translocation (Philadelphia chromosome) in CML patients and monitoring |
Bone marrow or peripheral blood |
Collect 1.0mL of BM or 3.0 mL of PB in EDTA (purple-top) tube |
Keep at room temp |
Ship overnight at room temperature |
Quantitation of e1a2 bcr-abl transcripts (Minor Breakpoint Region) by Real-Time PCR (14 days) |
Confirm suspected diagnosis of Acute Lymphoblastic Leukemia; monitoring of minimal residual disease (with chemotherapies and after bone marrow transplantation |
Bone marrow or peripheral blood |
Collect 1.0mL of BM or 3.0 mL of PB in EDTA (purple-top) tube |
Keep at room temp |
Ship overnight at room temperature |
Engraftment Baseline (10 days after receipt of Follow-up specimen) |
Determine recipient genotype prior to bone marrow transplant |
Bone marrow or peripheral blood |
Collect 1.0mL of BM or 3.0 mL of PB in EDTA (purple-top) tube |
Refrigerate sample 4° C until shipped |
Ship on cold packs to maintain temperature |
Buccal swab |
Swab inner facial cheek |
Seal in swab storage envelope and keep at room temp |
Ship at room temperature |
Engraftment Donor (10 days after receipt of Follow-up specimen) |
Determine donor genotype prior to bone marrow transplant |
Bone marrow or peripheral blood |
Collect 1.0mL of BM or 3.0 mL of PB in EDTA (purple-top) tube |
Refrigerate sample 4° C until shipped |
Ship on cold packs to maintain temperature |
Buccal swab |
Swab inner facial cheek |
Seal in swab storage envelope and keep at room temp |
Ship at room temperature |
Engraftment Follow-up (10 days) |
Monitoring for chimerism state following bone marrow engraftment |
Bone marrow or peripheral blood |
Collect 1.0mL of BM or 3.0 mL of PB in EDTA (purple-top) tube |
Refrigerate sample 4° C until shipped |
Ship on cold packs to maintain temperature |
T-Cell Engraftment Follow-up
(10 days) |
Monitoring for chimerism state following bone marrow engraftment using isolated T cells |
Peripheral blood |
Collect 3.0 mL of PB in EDTA (purple-top) tube. Samples should only be collected Monday – Thursday. |
Refrigerate sample 4° C until shipped. T cells must be isolated within 24 hours of collection. |
Ship on cold packs to maintain temperature |
JAK2 by PCR (14 days) |
Confirm diagnosis of Polycythemia Vera in patients with increased red cell mass and a low serum erythropoietin level |
Peripheral blood |
Collect 3.0 mL of PB in EDTA (purple-top) tube |
Refrigerate sample at 4° C until shipped |
Ship on cold packs to maintain temperature |
B & T-cell clonality by PCR (14 days) |
Determination of clonality of B and/or T cells in lymphomas and leukemias |
Bone marrow or peripheral blood |
Collect 1.0mL of BM or 3.0 mL of PB in EDTA (purple -top) or ACD (yellow-top) tube |
Refrigerate sample 4° C until shipped |
Ship on cold packs to maintain temperature |
Frozen tissue |
Biopsy or excise sample |
Snap freeze and hold in liquid nitrogen |
Ship on dry ice |
t (14;18) translocation by PCR
(21 days) |
Determination of t(14;18) translocation in B-cell lymphoma; diagnosis of follicular lymphoma, minimal residual disease |
Paraffin section |
None |
Cut 2-6 ribbons of 15-20 sections 5 µm thick; roll up and place into screw-top tubes provided by MDx lab |
Ship in screw top tubes provided by MDx lab |
B & T-cell gene rearrangement by Southern blot hybridization analysis (25 days) |
Determination of monoclonal rearrangement of B-cell IgH or T-cell surface receptors |
Frozen tissue |
Biopsy or excised sample |
Snap freeze and hold in liquid nitrogen |
Ship on dry ice |
Bone marrow or peripheral blood |
Collect 1.0 mL of BM or 3.0 mL of PB in EDTA (purple-top) or ACD (yellow-top) tube |
Refrigerate sample at 4° C |
Ship on cold packs to maintain temperature |
TEST NAME & TAT |
CLINICAL CONDITION |
SPECIMEN TYPE |
COLLECTION |
PROCESSING |
SHIPPING |
BK Virus using real-time PCR (14 days) |
Follow-up to kidney transplant |
Urine or peripheral blood |
10mL plastic tube (no additives) of urine; and/or 3.0 mL EDTA (purple-top) tube of peripheral blood |
For urine |
Deliver immediately (within 30 minutes) to lab so that the urine and/or plasma can be frozen until analysis |
CMV quantitative (7 days) |
Monitoring of CMV viremia in immunocompromised patients
Diagnosis of CMV disease |
Peripheral blood |
Collect 3.0 mL of PB in EDTA (purple-top) or PPT (pearl-top) tube |
Spin down and pipet off plasma within 4 hrs of collection; place plasma in freezer at -20° C |
Ship frozen on dry ice |
Epstein Barr Virus (EBV) Quantitative by PCR
(10 days) |
Diagnosis and monitoring of patients with Post-Transplant Lymphoproliferative Disorder (PTLD) |
Peripheral blood |
Collect 3.0 mL of PB in EDTA (purple-top) or PPT (pearl-top) tube |
Spin down and pipet off plasma within 4 hrs of collection; place plasma in freezer at -20° C |
Ship frozen on dry ice |
HBV quantitative by PCR
(21 days) |
Determine ongoing versus resolved HBV infection; monitor response to antiviral therapy |
Peripheral blood |
Collect 3.0 mL of PB in EDTA (purple-top) or PPT (pearl-top) tube |
Spin down and pipet off plasma within 4 hrs of collection; place plasma in freezer at -20° C |
Ship frozen on dry ice |
HCV quantitative by PCR
(14 days) |
Monitoring of viremia in patients diagnosed with HCV infection and determination of genotype |
Peripheral blood |
Collect 3.0 mL of PB in EDTA (purple-top) or PPT (pearl-top) tube |
Spin down and pipet off plasma within 4 hrs of collection; place serum in freezer at -20° C |
Ship frozen on dry ice; Contact 828-7284 for pickup or shipping details |
HCV genotyping (17 days)
|
HIV quantitative by PCR
(14 days) |
Monitoring of viremia in patients diagnosed with HIV infection and identification of antiretroviral drug resistance mutations |
Peripheral blood |
Collect 6.0 mL of PB in EDTA (pink-top) or PPT (pearl-top) tube |
Spin down and pipet off plasma within 4 hrs of collection; place plasma in freezer at -20° C |
Ship frozen on dry ice; Contact 828-7284 for pickup or shipping details |
HIV genotyping (14 days) |
Herpes Simplex Virus (HSV) in Cerebrospinal fluid (CSF) (7 days) |
Diagnosis and monitoring in neuropathy when HSV is suspected cause |
CSF |
A separate tube of CSF should be drawn if at all possible to prevent contamination of the sample |
|
Deliver immediately to lab so that it can be frozen until analysis |
TEST NAME & TAT |
CLINICAL CONDITION |
SPECIMEN TYPE |
COLLECTION |
PROCESSING |
SHIPPING |
CF Carrier Screening
(14 days) |
Identify inherited cause of Cystic Fibrosis that affects the respiratory tract, pancreas, intestines, reproductive hepatobiliary system and abnormally thick mucus that could lead to pneumonia, diarrhea, poor growth and infertility. |
Peripheral blood |
Collect 3.0 mL of PB in EDTA (purple-top tube) |
Refrigerate sample at 4° C until shipped |
Ship on cold packs to maintain temperature |
Factor V Leiden
(10 days) |
Determine cause of familial or personal history of hypercoagulability |
Peripheral blood |
Collect 1 full citrate (blue top) or EDTA (purple top) tube of peripheral blood |
Refrigerate sample at 4° C until shipped |
Ship on cold packs to maintain temperature |
Fragile X testing
(21 days) |
Determine fragile X gene mutation as cause or potential cause of mental retardation |
Peripheral blood |
Collect 3.0 mL of PB in EDTA (purple-top tube) |
Refrigerate sample at 4° C until shipped |
Ship on cold packs to maintain temperature |
Hereditary pancreatitis
(21 days) |
Identify inherited cause of pancreatitis |
Peripheral blood |
Collect 3.0 mL of PB in EDTA (purple-top) tube |
Refrigerate sample at 4° C until shipped |
Ship on cold packs to maintain temperature |
Prader-Willi/Angelman syndrome (21 days) |
Identify inherited cause of mental retardation |
Peripheral blood |
Collect 3.0 mL of PB in EDTA (purple-top) tube |
Refrigerate sample at 4° C until shipped |
Ship on cold packs to maintain temperature |
Prothrombin G20210A
(10 days) |
Determine cause of familial or personal history of hypercoagulability |
Peripheral blood |
Collect 1 full citrate (blue top) or EDTA (purple top) tube of peripheral blood |
Refrigerate sample at 4° C until shipped |
Ship on cold packs to maintain temperature |
TEST NAME & TAT |
CLINICAL CONDITION |
SPECIMEN TYPE |
COLLECTION |
PROCESSING |
SHIPPING |
Beta-2 Adrenergic Receptor genotyping (10 days) |
Optimize therapy of asthma patients with poor symptom control |
Peripheral blood |
Collect 3.0 mL of PB in EDTA (purple-top) or citrate (blue-top) tube. |
Refrigerate sample at 4° C until shipped |
Ship on cold packs to maintain temperature |
Warfarin Genotyping
(4 – 7 days) |
Determining maintenance dosage for patients on oral anticoagulant (Warfarin) used for the prophylaxis of thromboembolic episodes. |
Peripheral blood |
Collect 3.0 mL of PB in EDTA (purple-top) tube |
Refrigerate sample at 4° C until shipped |
Ship on cold packs to maintain temperature |
