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neuropathology services

Open Monday-Friday 8am-5pm 
Call Client Services 804-828-PATH (828-7284); 1-800-363-9234

Neuropathology Services offers expert neuro-biopsy interpretation. In addition, consultative services Nitya R. Ghatak, MD are available for brain, pituitary, muscle, nerve and eye biopsies, as well as for autopsy material. Supportive services include:

  • immunohistochemistry
  • electron microscopy (EM)
  • muscle histochemistry

Tests Performed

  • Brain biopsies and autopsies
    • Intraoperative diagnosis by H&E stained, touch prep, squash prep and frozen sections
    • Paraffin-embedded sections, H&E routine stain
    • Special stains--Bielschowsky, Luxol Fast Blue-PAS, Congo Red, Reticulin, other.
    • Immunohistochemistry--GFAP, S100, Synaptophysin, Chromogranin, EMA, CK, M1B-1, Beta amyloid, Tau, Ubiquitin, and others
    • EM--available if required
  • Pituitary biopsies
    • Intraoperative diagnosis
    • Paraffin sections--H&E and special stains
    • Immunohistochemistry (pituitary panel), ACTH, FSH, GH, LH, Prolactin, TSH
    • EM
  • Muscle biopsies
    • Paraffin sections--H&E and special stains if needed
    • Frozen section--H&E, Masson Trichrome, Adenosine Triphosphatase (ATPase) (acid and alkaline), NADH, PAS, Oil red O, Myophosphorylase
    • Immunohistochemistry (Paraffin and Frozen sections), Dystrophin, Myosin, and others
Click thumbnails to enlarge:

NADH muscle biopsy, frozen section

ATPase (alkaline) muscle biopsy, frozen section Dystrophin immunostaining muscle biopsy, paraffin embedded

Myosin immunostaining muscle biopsy, paraffin embedded

  • Nerve biopsies
    • Paraffin sections--H&E, LFB-PAS, Bielschowsky
    • Semi-thin sections from resin-embedded specimen
    • EM
    • Immunohistochemistry--CD68, CD45 (LCA), CD20, CD3,  and others
  • Ophthalmic Pathology
    • H&E, PAS, other special stains or procedures if needed

Specimen Handling Client Services 804-828-PATH (828-7284); 1-800-363-9234
Please refer to our Lexicomp Directory under Anatomical and Surgical Pathology for specimen handling information. 

Special Instructions
 A surgical pathology Accession Sheet (Adobe .pdf file Accession Sheet) with adequate clinical history and a differential diagnosis must accompany the specimens for their proper interpretation. Please include the proper ICD-9 code.

  1. All specimens are to be sent in 10% Formalin EXCEPT muscle for frozen section and nerve biopsies.
  2. Muscle biopsies must be scheduled at least 24-36 hours in advance.
  3. Muscle and nerve biopsies are to be delivered wrapped in saline-moistened gauze. If possible, they are NOT to be clamped.
  4. Must be delivered as soon as possible.

Please contact Client Services 804-828-PATH (828-7284); 1-800-363-9234 for assistance with the Accession Sheet and detailed information about transportation, specimen handling, separation prior to transportation and causes for rejection.

The Anatomic Pathology Receiving area is located at the MCV campus in the Gateway Building, 1200 E. Marshall Street, 6th floor, (804) 828-9739.

Consultative Services 
Consultative services are available for brain, pituitary, muscle, nerve and eye biopsies, as well as for autopsy material.

Education and Training 
All faculty members actively participate in numerous interdisciplinary conferences and are involved in the education of medical students and in the training of pathology, neurology, and neurosurgery residents. Residents complete a one-month rotation with access to extensive teaching materials including a Neuropathology CD-ROM, CAP Neuropathology survey cases, and selected glass slides. They attend slide conferences, clinico-pathological conferences, weekly brain cutting sessions, and participate in daily discussions of clinical cases.

The Department also offers a fellowship training program with the aim of preparing participants for neuropathology board certification. 


Updated March 3, 2008